: Beyond fluorescence, IFC provides data on cell size, shape, circularity, and internal complexity.
: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100. Imaging Flow Cytometry: Methods and Protocols
While IFC shares many preparatory steps with conventional flow cytometry, it requires specific optimizations to ensure high-quality imagery. : Beyond fluorescence, IFC provides data on cell